PEDF is an antifibrosis factor that inhibits the activation of fibroblasts in a bleomycin-induced pulmonary fibrosis rat model

• Main Authors: Chao, Z. (Author), Chen, J. (Author), Dong, H. (Author), Jia, C. (Author), Liang, J. (Author), Liu, X. (Author), Liu, Z. (Author), Qin, H. (Author), Qin, X. (Author), Yuan, Y. (Author), Zhang, H. (Author), Zhang, Z. (Author)
• Format: Article
• Language: English
• Published: BioMed Central Ltd 2022
• Subjects: animal; Animals; bleomycin; Bleomycin; C57BL mouse; eye protein; Eye Proteins; fibroblast; Fibroblast; Fibroblasts; fibrosing alveolitis; fibrosis; Fibrosis; Idiopathic Pulmonary Fibrosis; IPF; lung; Lung; metabolism; Mice; Mice, Inbred C57BL; mouse; nerve growth factor; Nerve Growth Factors; PEDF; peroxisome proliferator activated receptor; Peroxisome Proliferator-Activated Receptors; pigment epithelium-derived factor; PPAR-γ; rat; Rats; serine proteinase inhibitor; Serpins; transforming growth factor beta1; Transforming Growth Factor beta1
• Online Access: View Fulltext in Publisher
• ISBN: 14659921 (ISSN)
• DOI: 10.1186/s12931-022-02027-4

Background: Idiopathic pulmonary fibrosis (IPF) is a highly heterogeneous and fatal lung disease. In addition to dense fibrous tissue, abnormal angiogenesis is also an important feature of IPF. Pigment epithelium-derived factor (PEDF) is an angiogenesis inhibitor and a potential anti-fibrous factor. The purpose of this experiment is to observe the effect of PEDF on bleomycin (BLM)-induced pulmonary fibrosis in rats. Methods: In vivo, pathological examination and detection of related factors were performed on pulmonary fibrosis induced by BLM in rats, and the temporal and spatial distribution of PEDF was investigated. Furthermore, lung gene delivery (PEDF-adeno-associated virus) was performed to investigate the effect of PEDF on pulmonary fibrosis. In vitro, lentiviral vectors were used to construct PEDF over-expression or knock out primary rat lung (PRL) fibroblasts. The effect of PEDF on fibroblast activation under TGF-β1 stimulation was evaluated, and the activation of TGF-β1/smad pathway and PPAR-γ expression (in the presence or absence of PPAR-γ inhibitors) were analyzed. Results: In vivo results showed that PEDF expression decreased during the inflammatory phase and increased during the fibrotic phase. PEDF could inhibit the progression of pulmonary fibrosis in rats. In vitro results showed that PEDF could effectively inhibit TGF-β1-stimulated fibroblast activation and reduce the production of α-SMA and collagen-I. PEDF could inhibit the TGF-β1/smad pathway by up-regulating the activity of PPAR-γ. Conclusions: PEDF can act as an anti-fibrotic factor, inhibit fibroblast activation by upregulating PPAR-γ activity and reduce BLM-induced pulmonary fibrosis in rats. © 2022, The Author(s).