Active secretion of a thermostable transglutaminase variant in Escherichia coli
Background: Streptomyces mobaraenesis transglutaminase (smTG) is widely used to generate protein crosslinking or attachment of small molecules. However, the low thermostability is a main obstacle for smTG application. In addition, it is still hard to achieve the secretory expression of active smTG i...
Main Authors: | , , , , , , , , , |
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Format: | Article |
Language: | English |
Published: |
BioMed Central Ltd
2022
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Subjects: | |
Online Access: | View Fulltext in Publisher |