Colorimetric LPMO assay with direct implication for cellulolytic activity

Colorimetric LPMO assay with direct implication for cellulolytic activity

Background: Lytic polysaccharide monooxygenases (LPMOs) are important industrial enzymes known for their catalytic degradation of recalcitrant polymers such as cellulose or chitin. Their activity can be measured by lengthy HPLC methods, while high-throughput methods are less specific. A fast and spe...

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Bibliographic Details
Main Authors: Bertelsen, A.B (Author), Brander, S. (Author), Falkenberg, K.B (Author), Ipsen, J.Ø (Author), Johansen, K.S (Author), Lausten, S. (Author), Nørholm, M.H.H (Author), Østergaard, L.H (Author)
Format: Article
Language:English
Published: BioMed Central Ltd 2021
Subjects:
bioassay
Biochemical characterization
Catalysis
Catalysts
Catalytic degradation
cellulose
Cellulose
Cellulose degradation
Cellulose Degradation
chemical compound
concentration (composition)
Dehydroascorbate
enzyme activity
Enzyme activity
Enzyme assay
Enzyme characterization
Fructose
High throughput
High throughput screening
High-throughput assays
High-throughput method
Lytic polysaccharide monooxygenase
Oxidation
Phenolphthalein
polysaccharide
substrate
Substrates
Thermoascus aurantiacus
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