Improvements of the productivity and saccharification efficiency of the cellulolytic β-glucosidase D2-BGL in Pichia pastoris via directed evolution

Improvements of the productivity and saccharification efficiency of the cellulolytic β-glucosidase D2-BGL in Pichia pastoris via directed evolution

Background: β-Glucosidases are essential for cellulose hydrolysis by catalyzing the final cellulolytic degradation of cello-oligomers and cellobiose to glucose. D2-BGL is a fungal glycoside hydrolase family 3 (GH3) β-glucosidase isolated from Chaetomella raphigera with high substrate affinity, and i...

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Bibliographic Details
Main Authors: Ho, T.-H.D (Author), Kao, M.-R (Author), Yu, S.-M (Author)
Format: Article
Language:English
Published: BioMed Central Ltd 2021
Subjects:
amino acid
Amino acids
Amino Acids
Biochemical engineering
biomass
Biotechnology
catalysis
Catalytic efficiencies
cell
Cellulolytic enzyme
cellulose
Cellulose derivatives
Cellulose Derivatives
Cellulose hydrolysis
Chaetomella raphigera
Directed evolution
Efficiency
enzyme activity
Enzyme inhibition
GH3
glucose
Glycoside hydrolases
Hypocrea jecorina
Lignocellulosic biomass
Pichia pastoris
polymerase chain reaction
Productivity
protein
Protein engineering
Recombinant enzymes
Recombinant proteins
Saccharification
Saccharomyces cerevisiae
Substrate inhibition
Substrates
Unfolded protein response
Yeast
β-Glucosidase
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