CRISPR/Cas9-mediated genome editing directed by a 5S rRNA–tRNAGly hybrid promoter in the thermophilic filamentous fungus Humicola insolens

CRISPR/Cas9-mediated genome editing directed by a 5S rRNA–tRNAGly hybrid promoter in the thermophilic filamentous fungus Humicola insolens

Background: Humicola insolens is a filamentous fungus with high potential of producing neutral and heat- and alkali-resistant cellulase. However, the genetic engineering tools, particularly the genome-editing tool, are scarce, hindering the study of cellulase expression regulation in this organism....

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Bibliographic Details
Main Authors: Fan, C. (Author), Huang, H. (Author), Ji, W. (Author), Liu, B. (Author), Luo, H. (Author), Su, X. (Author), Xu, X. (Author), Yao, B. (Author), Zhang, W. (Author), Zhang, Y. (Author)
Format: Article
Language:English
Published: BioMed Central Ltd 2021
Subjects:
5s rRNA
5S rRNA
biofuel
biotechnology
Cellulase
CRISPR/cas9
CRISPR/Cas9
experimental study
Expression regulation
Filamentous fungus
gene expression
Genetic engineering
Hepatitis delta virus
High potential
Humicola insolens
Hybrid promoter
laboratory method
RNA
Thermophilic filamentous fungus
Transcription
tRNAGly
TRNAgly
Viruses
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