Tunnel engineering for modulating the substrate preference in cytochrome P450BsβHI
An active site is normally located inside enzymes, hence substrates should go through a tunnel to access the active site. Tunnel engineering is a powerful strategy for refining the catalytic properties of enzymes. Here, P450BsβHI (Q85H/V170I) derived from hydroxylase P450Bsβ from Bacillus subtilis w...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Springer Science and Business Media Deutschland GmbH
2021
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| Subjects: | |
| Online Access: | View Fulltext in Publisher |