Engineering gene overlaps to sustain genetic constructs in vivo

Engineering gene overlaps to sustain genetic constructs in vivo

Evolution is often an obstacle to the engineering of stable biological systems due to the selection of mutations inactivating costly gene circuits. Gene overlaps induce important constraints on sequences and their evolution. We show that these constraints can be harnessed to increase the stability o...

Full description

Bibliographic Details
Main Authors: Bernheim, A. (Author), Decrulle, A.L (Author), Frénoy, A. (Author), Gutierrez, A. (Author), Lindner, A.B (Author), Lotton, C. (Author), Meiller-Legrand, T.A (Author)
Format: Article
Language:English
Published: Public Library of Science 2021
Subjects:
algorithm
Algorithms
article
DNA sequence
Escherichia coli
essential gene
Evolution, Molecular
Genes, Essential
genetic engineering
Genetic Engineering
genetics
genomics
Genomics
in vivo study
loss of function mutation
molecular evolution
mutation
Mutation
natural selection
nonhuman
overlapping gene
procedures
reading frame
Reading Frames
Sequence Analysis, DNA
synthetic biology
Synthetic Biology
Online Access:View Fulltext in Publisher

Internet

View Fulltext in Publisher

Similar Items