Time to revisit the endpoint dilution assay and to replace the TCID50 as a measure of a virus sample’s infection concentration

Time to revisit the endpoint dilution assay and to replace the TCID50 as a measure of a virus sample’s infection concentration

The endpoint dilution assay’s output, the 50% infectious dose (ID50), is calculated using the Reed-Muench or Spearman-Kärber mathematical approximations, which are biased and often miscalculated. We introduce a replacement for the ID50 that we call Specific INfection (SIN) along with a free and ope...

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Bibliographic Details
Main Authors: Beauchemin, C.A.A (Author), Cresta, D. (Author), Lane, L.C (Author), Quirouette, C. (Author), Smith, A.M (Author), Smith, A.P (Author), Warren, D.C (Author)
Format: Article
Language:English
Published: Public Library of Science 2021
Subjects:
Article
Bayes theorem
Bayes Theorem
bioassay
Biological Assay
biology
Computational Biology
controlled study
dilution
endpoint dilution assay
focus forming assay
Human respiratory syncytial virus
ID50 (median infectious dose)
in vitro study
Influenza virus
intermethod comparison
measurement accuracy
multiplicity of infection
nonhuman
plaque forming unit
procedures
reliability
TCID50
viral plaque assay
Viral Plaque Assay
virology
virus concentration
Virus Diseases
virus infection
virus infectivity
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