Optimized RNA isolation of FFPE uterine scar tissues for RNA expression analyses delineated by laser microdissection

Optimized RNA isolation of FFPE uterine scar tissues for RNA expression analyses delineated by laser microdissection

Samples for histological analyses are often formalin-fixed paraffin-embedded (FFPE) and slide-mounted, which complicates RNA extraction for many downstream molecular applications. Furthermore, when the region of interest is extremely small due to isolation with laser microdissection (LMD), extractin...

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Bibliographic Details
Main Authors: Basler, C. (Author), Braun, T. (Author), C Rancourt, R. (Author), Ehrlich, L. (Author), Henrich, W. (Author), Melchior, K. (Author), Paping, A. (Author)
Format: Article
Language:English
Published: NLM (Medline) 2022
Subjects:
Cicatrix
FFPE
formaldehyde
Formaldehyde
genetics
human
Humans
laser
laser capture microdissection
Laser Capture Microdissection
laser microdissection
Lasers
LMD
paraffin
Paraffin Embedding
procedures
qRT-PCR
RNA
RNA expression
RNA isolation
scar
tissue fixation
Tissue Fixation
uterine scar tissue
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