Advancing the Rose Rosette Virus Minireplicon and Encapsidation System by Incorporating GFP, Mutations, and the CMV 2b Silencing Suppressor

Advancing the Rose Rosette Virus Minireplicon and Encapsidation System by Incorporating GFP, Mutations, and the CMV 2b Silencing Suppressor

Plant infecting emaraviruses have segmented negative strand RNA genomes and little is known about their infection cycles due to the lack of molecular tools for reverse genetic studies. Therefore, we innovated a rose rosette virus (RRV) minireplicon containing the green fluorescent protein (GFP) gene...

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Bibliographic Details
Main Authors: Romay, G. (Author), Shaw, B.D (Author), Urrutia, C.D (Author), Verchot, J. (Author)
Format: Article
Language:English
Published: NLM (Medline) 2022
Subjects:
bunyavirus
cytomegalovirus infection
Cytomegalovirus Infections
DNA virus
DNA Viruses
emaravirus
envelope glycoproteins
genetics
green fluorescent protein
Green Fluorescent Proteins
guide RNA
minireplicon
mutation
Mutation
negative strand RNA virus
plant disease
Plant Diseases
plant virus
reverse genetic systems
RNA, Guide
RNA, Viral
RNA-Dependent RNA Polymerase
Rosa
rose
rose rosette virus
virion assembly
virus forms
virus replication
virus RNA
Viruses, Unclassified
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