A Mobile Laboratory Enables Fecal Pollution Source Tracking in Catchments Using Onsite qPCR Assays

A Mobile Laboratory Enables Fecal Pollution Source Tracking in Catchments Using Onsite qPCR Assays

Onsite molecular diagnostics can revolutionize fecal pollution source tracking. We aimed to validate a method for onsite qPCR assays with a miniature speaker-sized Q qPCR instrument and other portable equipment items. We showed that marker genes for total bacteria (16S) and E. coli (rodA) in 100 mL...

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Bibliographic Details
Main Authors: Acharya, K. (Author), Blackburn, A. (Author), Kilsby, C.G (Author), Werner, D. (Author), Zan, R. (Author)
Format: Article
Language:English
Published: MDPI 2022
Subjects:
16S rRNA gene
Catchments
Effluents
Escherichia coli
Field kit
field kits
Genes
Hf183 gene
HF183 gene
lab in a van
Lab in a van
Laboratories
Lakes
microbial source tracking
Microbial source tracking
Mobile laboratories
mobile laboratory
Polymerase chain reaction
portable qPCR
Portable qPCR
Principal component analysis
river pollution
River pollution
River water
Rivers
RNA
rodA gene
Roda gene
Storms
stormwater
Stormwaters
Water quality
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