Genetic Transformation of a Clinical (Genital Tract), Plasmid-Free Isolate of Chlamydia trachomatis: Engineering the Plasmid as a Cloning Vector

Our study had three objectives: to extend the plasmid-based transformation protocol to a clinical isolate of C. trachomatis belonging to the trachoma biovar, to provide "proof of principle" that it is possible to "knock out" selected plasmid genes (retaining a replication compete...

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Bibliographic Details
Main Authors: Wang, Yibing (Author), Kahane, Simona (Author), Cutcliffe, Lesley T. (Author), Skilton, Rachel J. (Author), Lambden, Paul R. (Author), Persson, Kenneth (Author), Bjartling, Carina (Author), Clarke, Ian N. (Author)
Format: Article
Language:English
Published: 2013.
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