Genetic Transformation of a Clinical (Genital Tract), Plasmid-Free Isolate of Chlamydia trachomatis: Engineering the Plasmid as a Cloning Vector
Our study had three objectives: to extend the plasmid-based transformation protocol to a clinical isolate of C. trachomatis belonging to the trachoma biovar, to provide "proof of principle" that it is possible to "knock out" selected plasmid genes (retaining a replication compete...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
2013.
|
Subjects: | |
Online Access: | Get fulltext |