Single-channel electrophysiology of cell-free expressed ion channels by direct incorporation in lipid bilayers
Single-channel electrophysiology with lipid bilayer systems requires ion channel expression, purification from cell culture, and reconstitution in proteoliposomes for delivery to a planar bilayer. Here we demonstrate that single-channel current measurements of the potassium channels KcsA and hERGS5-...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
2013-10-18.
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| Subjects: | |
| Online Access: | Get fulltext Get fulltext |
| LEADER | 01176 am a22002053u 4500 | ||
|---|---|---|---|
| 001 | 359046 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Friddin, Mark S. |e author |
| 700 | 1 | 0 | |a Smithers, Natalie P. |e author |
| 700 | 1 | 0 | |a Beaugrand, Maïwenn |e author |
| 700 | 1 | 0 | |a Marcotte, Isabelle |e author |
| 700 | 1 | 0 | |a Williamson, Philip T.F. |e author |
| 700 | 1 | 0 | |a Morgan, Hywel |e author |
| 700 | 1 | 0 | |a de Planque, Maurits R.R. |e author |
| 245 | 0 | 0 | |a Single-channel electrophysiology of cell-free expressed ion channels by direct incorporation in lipid bilayers |
| 260 | |c 2013-10-18. | ||
| 856 | |z Get fulltext |u https://eprints.soton.ac.uk/359046/1/Friddin_Analyst_2014.pdf | ||
| 856 | |z Get fulltext |u https://eprints.soton.ac.uk/359046/2/Friddin_Analyst_2014sup.pdf | ||
| 520 | |a Single-channel electrophysiology with lipid bilayer systems requires ion channel expression, purification from cell culture, and reconstitution in proteoliposomes for delivery to a planar bilayer. Here we demonstrate that single-channel current measurements of the potassium channels KcsA and hERGS5-S6 can be obtained by direct insertion in interdroplet lipid bilayers from microliters of a cell-free expression medium. | ||
| 655 | 7 | |a Article | |