Triplex-mediated analysis of cytosine methylation at CpA sites in DNA

Triplex-mediated analysis of cytosine methylation at CpA sites in DNA

Modified triplex-forming oligonucleotides distinguish 5-methyl cytosine from unmethylated cytosine in DNA duplexes by differences in triplex melting temperatures. The discrimination is sequence-specific; dramatic differences in stabilisation are seen for CpA methylation, whereas CpG methylation is n...

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Bibliographic Details
Main Authors: Johannsen, Marie W. (Author), Gerrard, Simon R. (Author), Melvin, Tracy (Author), Brown, Tom (Author)
Format: Article
Language:English
Published: 2014-01-18.
Subjects:
Article
Online Access:Get fulltext
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100 1 0 |a Johannsen, Marie W.  |e author 
700 1 0 |a Gerrard, Simon R.  |e author 
700 1 0 |a Melvin, Tracy  |e author 
700 1 0 |a Brown, Tom  |e author 
245 0 0 |a Triplex-mediated analysis of cytosine methylation at CpA sites in DNA 
260 |c 2014-01-18. 
856 |z Get fulltext  |u https://eprints.soton.ac.uk/360139/1/c3cc45917a 
520 |a Modified triplex-forming oligonucleotides distinguish 5-methyl cytosine from unmethylated cytosine in DNA duplexes by differences in triplex melting temperatures. The discrimination is sequence-specific; dramatic differences in stabilisation are seen for CpA methylation, whereas CpG methylation is not detected. This direct detection of DNA methylation constitutes a new approach for epigenetic analysis. 
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