A mutant of uracil DNA glycosylase that distinguishes between cytosine and 5-methylcytosine
We demonstrate that a mutant of uracil DNA glycosylase (N123D:L191A) distinguishes between cytosine and methylcytosine. Uracil DNA glycosylase (UDG) efficiently removes uracil from DNA in a reaction in which the base is flipped into the enzyme's active site. Uracil is selected over cytosine by...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
2014-04-16.
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| Subjects: | |
| Online Access: | Get fulltext |