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01718 am a22001693u 4500 |
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372860 |
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|a Saha, Shimul C.
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|a Powl, Andrew M.
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|a Wallace, B. A.
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|a de Planque, Maurits R.R.
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|a Morgan, Hywel
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|a Screening ion-channel ligand interactions with passive pumping in a microfluidic bilayer lipid membrane (BLM) chip
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|c 2015-01-09.
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|z Get fulltext
|u https://eprints.soton.ac.uk/372860/1/Saha2015.pdf
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|a We describe a scalable artificial bilayer lipid membrane (BLM) platform for rapid electrophysiological screening of ion channels and transporters. A passive pumping method is used to flow microliter volumes of ligand solution across a suspended bilayer within a microfluidic chip. Bilayers are stable at flow rates up to ?0.5 µL/min. Phospholipid bilayers are formed across a photolithographically defined aperture made in a dry film resist within the microfluidic chip. Bilayers are stable for many days and the low shunt capacitance of the thin film support gives low-noise high-quality single ion channel recording. Dose-dependent transient blocking of ?-hemolysin with ?-cyclodextrin (?-CD) and polyethylene glycol (PEG) is demonstrated and dose-dependent blocking studies of the KcsA potassium channel with tetraethylammonium (TEA) show the potential for determining IC50 values. The assays are fast (30 minutes for a complete IC50 curve) and simple and require very small amounts of compounds (100 ?g in 15 ?L). The technology can be scaled so that multiple bilayers can be addressed, providing a screening platform for ion channels, transporters and nanopores.
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