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10.1016-j.bbrc.2016.06.127 |
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|a Differential effects of two-pore channel protein 1 and 2 silencing in MDA-MB-468 breast cancer cells
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|c 2016
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|z View Fulltext in Publisher
|u https://doi.org/10.1016/j.bbrc.2016.06.127
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|a Two-pore channel proteins, TPC1 and TPC2, are calcium permeable ion channels found localized to the membranes of endolysosomal calcium stores. There is increasing interest in the role of TPC-mediated intracellular signaling in various pathologies; however their role in breast cancer has not been extensively evaluated. TPC1 and TPC2 mRNA was present in all non-tumorigenic and tumorigenic breast cell lines assessed. Silencing of TPC2 but not TPC1 attenuated epidermal growth factor-induced vimentin expression in MDA-MB-468 breast cancer cells. This effect was not due to a general inhibition of epithelial to mesenchymal transition (EMT) as TPC2 silencing had no effect on epidermal growth factor (EGF)-induced changes on E-cadherin expression. TPC1 and TPC2 were also shown to differentially regulate cyclopiazonic acid (CPA)-mediated changes in cytosolic free Ca2+. These findings indicate potential differential regulation of signaling processes by TPC1 and TPC2 in breast cancer cells. (C) 2016 Elsevier Inc. All rights reserved.
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|a ACTIVATION
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|a Breast cancer
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|a CA2+
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|a Calcium
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|a CALCIUM
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|a Calcium signaling
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|a ENTRY
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|a Epidermal growth factor
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|a EPITHELIAL-MESENCHYMAL TRANSITION
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|a GENE-EXPRESSION
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|a KINASES
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|a LINES
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|a NAADP
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|a Two-pore channel
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|a Vimentin
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|a Jahidin, AH
|e author
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|a Monteith, GR
|e author
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|a Roberts-Thomson, SJ
|e author
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|a Stewart, TA
|e author
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|a Thompson, EW
|e author
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|t BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
|g 477 4, 731-736
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