real time polymerase chain reaction 181 human 146 Article 139 Humans 129 controlled study 114 genetics 102 male 81 female 78 nonhuman 70 metabolism 67 adult 66 gene expression 65 Female 60 Male 58 unclassified drug 53 Real-Time Polymerase Chain Reaction 49 protein expression 47 middle aged 44 upregulation 43 Middle Aged 41 Western blotting 40 major clinical study 40 gene expression regulation 36 pathology 34 human cell 33 messenger RNA 33 human tissue 31 animal experiment 30 Animals 28 down regulation 28