Activation analysis Amino Acid Substitution Amino acid sequence Amino acids Animals C-terminal sequences Calcium Calcium Imaging Calcium Influx Cell death Channel Gating Current-voltage relationship HEK293 Cells Humans Ion Channel Gating Ion Channels Mice Models, Molecular Mutation, Missense Non-selective cation channels Open conformation Patch Clamp Peptide Mapping Phosphoinositides Protein Structure, Secondary Protein Structure, Tertiary Proteins Rats Signal Transduction Signaling cascades