A cost efficient protocol to introduce epitope tags by CRISPR-Cas9 mediated gene knock-in with asymmetric semi-double stranded template

by Qi Fang, Yoichi Shinkai
Published 2021-01-01

SETDB1-Mediated Silencing of Retroelements

by Kei Fukuda, Yoichi Shinkai
Published 2020-05-01

A Simple Method for Visualization of Locus-Specific H4K20me1 Modifications in Living Caenorhabditis elegans Single Cells

by Yoichi Shinkai, Masahiro Kuramochi, Motomichi Doi
Published 2018-07-01

New Family Members of FG Repeat Proteins and Their Unexplored Roles During Phase Separation

by Yoichi Shinkai, Masahiro Kuramochi, Takamitsu Miyafusa
Published 2021-07-01

A somatic role for the histone methyltransferase Setdb1 in endogenous retrovirus silencing

by Masaki Kato, Keiko Takemoto, Yoichi Shinkai
Published 2018-04-01

The fibronectin type-III (FNIII) domain of ATF7IP contributes to efficient transcriptional silencing mediated by the SETDB1 complex

by Takeshi Tsusaka, Kei Fukuda, Chikako Shimura, Masaki Kato, Yoichi Shinkai
Published 2020-11-01

Selenium-based S-adenosylmethionine analog reveals the mammalian seven-beta-strand methyltransferase METTL10 to be an EF1A1 lysine methyltransferase.

by Tadahiro Shimazu, Joaquin Barjau, Yoshihiro Sohtome, Mikiko Sodeoka, Yoichi Shinkai
Published 2014-01-01

Prdm8 regulates the morphological transition at multipolar phase during neocortical development.

by Mayuko Inoue, Takao Kuroda, Aya Honda, Mariko Komabayashi-Suzuki, Tae Komai, Yoichi Shinkai, Ken-ichi Mizutani
Published 2014-01-01

Regulation of mammalian 3D genome organization and histone H3K9 dimethylation by H3K9 methyltransferases

by Kei Fukuda, Chikako Shimura, Hisashi Miura, Akie Tanigawa, Takehiro Suzuki, Naoshi Dohmae, Ichiro Hiratani, Yoichi Shinkai
Published 2021-05-01

Uncharged Components of Single-Stranded DNA Modulate Liquid–Liquid Phase Separation With Cationic Linker Histone H1

by Masahiro Mimura, Masahiro Mimura, Shunsuke Tomita, Hiroka Sugai, Hiroka Sugai, Yoichi Shinkai, Sayaka Ishihara, Ryoji Kurita, Ryoji Kurita
Published 2021-08-01

Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly

by Atsuko Shirai, Takayuki Kawaguchi, Hideaki Shimojo, Daisuke Muramatsu, Mayumi Ishida-Yonetani, Yoshifumi Nishimura, Hiroshi Kimura, Jun-ichi Nakayama, Yoichi Shinkai
Published 2017-08-01

Correction: Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly

by Atsuko Shirai, Takayuki Kawaguchi, Hideaki Shimojo, Daisuke Muramatsu, Mayumi Ishida-Yonetani, Yoshifumi Nishimura, Hiroshi Kimura, Jun-ichi Nakayama, Yoichi Shinkai
Published 2017-09-01

Tri-methylation of ATF7IP by G9a/GLP recruits the chromodomain protein MPP8

by Takeshi Tsusaka, Masaki Kikuchi, Tadahiro Shimazu, Takehiro Suzuki, Yoshihiro Sohtome, Mai Akakabe, Mikiko Sodeoka, Naoshi Dohmae, Takashi Umehara, Yoichi Shinkai
Published 2018-10-01

Histone H3K9 Methyltransferase G9a in Oocytes Is Essential for Preimplantation Development but Dispensable for CG Methylation Protection

by Wan Kin Au Yeung, Julie Brind’Amour, Yu Hatano, Kazuo Yamagata, Robert Feil, Matthew C. Lorincz, Makoto Tachibana, Yoichi Shinkai, Hiroyuki Sasaki
Published 2019-04-01

Combined Loss of JMJD1A and JMJD1B Reveals Critical Roles for H3K9 Demethylation in the Maintenance of Embryonic Stem Cells and Early Embryogenesis

by Shunsuke Kuroki, Yuji Nakai, Ryo Maeda, Naoki Okashita, Mika Akiyoshi, Yutaro Yamaguchi, Satsuki Kitano, Hitoshi Miyachi, Ryuichiro Nakato, Kenji Ichiyanagi, Katsuhiko Shirahige, Hiroshi Kimura, Yoichi Shinkai, Makoto Tachibana
Published 2018-04-01

Derepression of inflammation-related genes link to microglia activation and neural maturation defect in a mouse model of Kleefstra syndrome

by Ayumi Yamada, Takae Hirasawa, Kayako Nishimura, Chikako Shimura, Naomi Kogo, Kei Fukuda, Madoka Kato, Masaki Yokomori, Tetsutaro Hayashi, Mana Umeda, Mika Yoshimura, Yoichiro Iwakura, Itoshi Nikaido, Shigeyoshi Itohara, Yoichi Shinkai
Published 2021-07-01

Inhibition of histone methyltransferase G9a attenuates liver cancer initiation by sensitizing DNA-damaged hepatocytes to p53-induced apoptosis

by Takuma Nakatsuka, Keisuke Tateishi, Hiroyuki Kato, Hiroaki Fujiwara, Keisuke Yamamoto, Yotaro Kudo, Hayato Nakagawa, Yasuo Tanaka, Hideaki Ijichi, Tsuneo Ikenoue, Takeaki Ishizawa, Kiyoshi Hasegawa, Makoto Tachibana, Yoichi Shinkai, Kazuhiko Koike
Published 2021-01-01

Histone H3 Methylated at Arginine 17 Is Essential for Reprogramming the Paternal Genome in Zygotes

by Yuki Hatanaka, Takeshi Tsusaka, Natsumi Shimizu, Kohtaro Morita, Takehiro Suzuki, Shinichi Machida, Manabu Satoh, Arata Honda, Michiko Hirose, Satoshi Kamimura, Narumi Ogonuki, Toshinobu Nakamura, Kimiko Inoue, Yoshihiko Hosoi, Naoshi Dohmae, Toru Nakano, Hitoshi Kurumizaka, Kazuya Matsumoto, Yoichi Shinkai, Atsuo Ogura
Published 2017-09-01

DMRT1-mediated reprogramming drives development of cancer resembling human germ cell tumors with features of totipotency

by Jumpei Taguchi, Hirofumi Shibata, Mio Kabata, Masaki Kato, Kei Fukuda, Akito Tanaka, Sho Ohta, Tomoyo Ukai, Kanae Mitsunaga, Yosuke Yamada, So I Nagaoka, Sho Yamazawa, Kotaro Ohnishi, Knut Woltjen, Tetsuo Ushiku, Manabu Ozawa, Mitinori Saitou, Yoichi Shinkai, Takuya Yamamoto, Yasuhiro Yamada
Published 2021-08-01

The methyltransferase METTL9 mediates pervasive 1-methylhistidine modification in mammalian proteomes

by Erna Davydova, Tadahiro Shimazu, Maren Kirstin Schuhmacher, Magnus E. Jakobsson, Hanneke L. D. M. Willemen, Tongri Liu, Anders Moen, Angela Y. Y. Ho, Jędrzej Małecki, Lisa Schroer, Rita Pinto, Takehiro Suzuki, Ida A. Grønsberg, Yoshihiro Sohtome, Mai Akakabe, Sara Weirich, Masaki Kikuchi, Jesper V. Olsen, Naoshi Dohmae, Takashi Umehara, Mikiko Sodeoka, Valentina Siino, Michael A. McDonough, Niels Eijkelkamp, Christopher J. Schofield, Albert Jeltsch, Yoichi Shinkai, Pål Ø. Falnes
Published 2021-02-01