LRGUK-1 is required for basal body and manchette function during spermatogenesis and male fertility.
Male infertility affects at least 5% of reproductive age males. The most common pathology is a complex presentation of decreased sperm output and abnormal sperm shape and motility referred to as oligoasthenoteratospermia (OAT). For the majority of OAT men a precise diagnosis cannot be provided. Here...
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2015-03-01
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Series: | PLoS Genetics |
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doaj-040121e1c3374ed8a427d065623bd48c2020-11-24T21:32:38ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042015-03-01113e100509010.1371/journal.pgen.1005090LRGUK-1 is required for basal body and manchette function during spermatogenesis and male fertility.Yan LiuKathleen DeBoerDavid M de KretserLiza O'DonnellAnne E O'ConnorD Jo MerrinerHidenobu OkudaBelinda WhittleDavid A JansAthina EfthymiadisRobert I McLachlanChristopher J OrmandyChris C GoodnowDuangporn JamsaiMoira K O'BryanMale infertility affects at least 5% of reproductive age males. The most common pathology is a complex presentation of decreased sperm output and abnormal sperm shape and motility referred to as oligoasthenoteratospermia (OAT). For the majority of OAT men a precise diagnosis cannot be provided. Here we demonstrate that leucine-rich repeats and guanylate kinase-domain containing isoform 1 (LRGUK-1) is required for multiple aspects of sperm assembly, including acrosome attachment, sperm head shaping and the initiation of the axoneme growth to form the core of the sperm tail. Specifically, LRGUK-1 is required for basal body attachment to the plasma membrane, the appropriate formation of the sub-distal appendages, the extension of axoneme microtubules and for microtubule movement and organisation within the manchette. Manchette dysfunction leads to abnormal sperm head shaping. Several of these functions may be achieved in association with the LRGUK-1 binding partner HOOK2. Collectively, these data establish LRGUK-1 as a major determinant of microtubule structure within the male germ line.http://europepmc.org/articles/PMC4363142?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yan Liu Kathleen DeBoer David M de Kretser Liza O'Donnell Anne E O'Connor D Jo Merriner Hidenobu Okuda Belinda Whittle David A Jans Athina Efthymiadis Robert I McLachlan Christopher J Ormandy Chris C Goodnow Duangporn Jamsai Moira K O'Bryan |
spellingShingle |
Yan Liu Kathleen DeBoer David M de Kretser Liza O'Donnell Anne E O'Connor D Jo Merriner Hidenobu Okuda Belinda Whittle David A Jans Athina Efthymiadis Robert I McLachlan Christopher J Ormandy Chris C Goodnow Duangporn Jamsai Moira K O'Bryan LRGUK-1 is required for basal body and manchette function during spermatogenesis and male fertility. PLoS Genetics |
author_facet |
Yan Liu Kathleen DeBoer David M de Kretser Liza O'Donnell Anne E O'Connor D Jo Merriner Hidenobu Okuda Belinda Whittle David A Jans Athina Efthymiadis Robert I McLachlan Christopher J Ormandy Chris C Goodnow Duangporn Jamsai Moira K O'Bryan |
author_sort |
Yan Liu |
title |
LRGUK-1 is required for basal body and manchette function during spermatogenesis and male fertility. |
title_short |
LRGUK-1 is required for basal body and manchette function during spermatogenesis and male fertility. |
title_full |
LRGUK-1 is required for basal body and manchette function during spermatogenesis and male fertility. |
title_fullStr |
LRGUK-1 is required for basal body and manchette function during spermatogenesis and male fertility. |
title_full_unstemmed |
LRGUK-1 is required for basal body and manchette function during spermatogenesis and male fertility. |
title_sort |
lrguk-1 is required for basal body and manchette function during spermatogenesis and male fertility. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Genetics |
issn |
1553-7390 1553-7404 |
publishDate |
2015-03-01 |
description |
Male infertility affects at least 5% of reproductive age males. The most common pathology is a complex presentation of decreased sperm output and abnormal sperm shape and motility referred to as oligoasthenoteratospermia (OAT). For the majority of OAT men a precise diagnosis cannot be provided. Here we demonstrate that leucine-rich repeats and guanylate kinase-domain containing isoform 1 (LRGUK-1) is required for multiple aspects of sperm assembly, including acrosome attachment, sperm head shaping and the initiation of the axoneme growth to form the core of the sperm tail. Specifically, LRGUK-1 is required for basal body attachment to the plasma membrane, the appropriate formation of the sub-distal appendages, the extension of axoneme microtubules and for microtubule movement and organisation within the manchette. Manchette dysfunction leads to abnormal sperm head shaping. Several of these functions may be achieved in association with the LRGUK-1 binding partner HOOK2. Collectively, these data establish LRGUK-1 as a major determinant of microtubule structure within the male germ line. |
url |
http://europepmc.org/articles/PMC4363142?pdf=render |
work_keys_str_mv |
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