Unstable Expression of Commonly Used Reference Genes in Rat Pancreatic Islets Early after Isolation Affects Results of Gene Expression Studies.

The use of RT-qPCR provides a powerful tool for gene expression studies; however, the proper interpretation of the obtained data is crucially dependent on accurate normalization based on stable reference genes. Recently, strong evidence has been shown indicating that the expression of many commonly...

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Bibliographic Details
Main Authors: Lucie Kosinová, Monika Cahová, Eva Fábryová, Irena Týcová, Tomáš Koblas, Ivan Leontovyč, František Saudek, Jan Kříž
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2016-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4817981?pdf=render