Overcoming the Refractory Expression of Secreted Recombinant Proteins in Mammalian Cells through Modification of the Signal Peptide and Adjacent Amino Acids.

The expression and subsequent purification of mammalian recombinant proteins is of critical importance to many areas of biological science. To maintain the appropriate tertiary structure and post-translational modifications of such proteins, transient mammalian expression systems are often adopted....

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Bibliographic Details
Main Authors: Gülin Güler-Gane, Sara Kidd, Sudharsan Sridharan, Tristan J Vaughan, Trevor C I Wilkinson, Natalie J Tigue
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2016-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4873207?pdf=render