Characterization and remediation of sample index swaps by non-redundant dual indexing on massively parallel sequencing platforms

Characterization and remediation of sample index swaps by non-redundant dual indexing on massively parallel sequencing platforms

Abstract Background Here we present an in-depth characterization of the mechanism of sequencer-induced sample contamination due to the phenomenon of index swapping that impacts Illumina sequencers employing patterned flow cells with Exclusion Amplification (ExAmp) chemistry (HiSeqX, HiSeq4000, and N...

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Bibliographic Details
Main Authors: Maura Costello, Mark Fleharty, Justin Abreu, Yossi Farjoun, Steven Ferriera, Laurie Holmes, Brian Granger, Lisa Green, Tom Howd, Tamara Mason, Gina Vicente, Michael Dasilva, Wendy Brodeur, Timothy DeSmet, Sheila Dodge, Niall J. Lennon, Stacey Gabriel
Format: Article
Language:English
Published: BMC 2018-05-01
Series:BMC Genomics
Subjects:
Next generation sequencing
Massively parallel sequencing
ILLUMINA sequencing
Index swapping
Index hopping
Multiplexing
Online Access:http://link.springer.com/article/10.1186/s12864-018-4703-0

Internet

http://link.springer.com/article/10.1186/s12864-018-4703-0

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