Wheat bran feruloyl oligosaccharides ameliorate AAPH-induced oxidative stress in HepG2 cells via Nrf2 signalling
Protective effects of wheat bran feruloyl oligosaccharides (FOs) against 2, 2′-Azobis (2-methylpropionamidine) dihydrochloride (AAPH)-induced oxidative stress in HepG2 cells were investigated. The cytoprotective effects of FOs against oxidative challenge were determined by cell viability, intracellu...
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doaj-2d3b96ae860f4c06835485a8612119ec2021-04-30T07:08:08ZengElsevierJournal of Functional Foods1756-46462016-08-0125333340Wheat bran feruloyl oligosaccharides ameliorate AAPH-induced oxidative stress in HepG2 cells via Nrf2 signallingHuijuan Zhang0Jing Wang1Yingli Liu2Lingxiao Gong3Baoguo Sun4Innovation Center for Food Nutrition and Human Health, Beijing Technology & Business University (BTBU), Beijing 100048, ChinaInnovation Center for Food Nutrition and Human Health, Beijing Technology & Business University (BTBU), Beijing 100048, China; Key Laboratory of Space Nutrition and Food Engineering, Beijing 100094, China; Tianjin Food Safety & Low Carbon Manufacturing Collaborative Innovation Center, Tianjin 300457, China; Corresponding author. School of Food Science and Chemical Engineering, Beijing Technology and Business University, No. 11, Fucheng Road, Haidian District, Beijing 100048, China. Tel.: +86 010 68985378; fax: +86 010 68985378.Innovation Center for Food Nutrition and Human Health, Beijing Technology & Business University (BTBU), Beijing 100048, ChinaInnovation Center for Food Nutrition and Human Health, Beijing Technology & Business University (BTBU), Beijing 100048, ChinaInnovation Center for Food Nutrition and Human Health, Beijing Technology & Business University (BTBU), Beijing 100048, ChinaProtective effects of wheat bran feruloyl oligosaccharides (FOs) against 2, 2′-Azobis (2-methylpropionamidine) dihydrochloride (AAPH)-induced oxidative stress in HepG2 cells were investigated. The cytoprotective effects of FOs against oxidative challenge were determined by cell viability, intracellular reactive oxygen species (ROS) generation, and lactate dehydrogenase (LDH) leakage. The antioxidant activities of FOs were investigated by determining the activities and mRNA expression levels of phase II detoxifying/antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx). FOs concentrations had a positive correlation with cell viability and a negative correlation with the LDH activity. FOs treatment increased the activities and mRNA levels of SOD, CAT, and GPx. Moreover, the nuclear factor E2 related factor (Nrf2) protein expression in the FOs group positively correlated with the SOD, CAT, and GPx protein levels. Thus the results demonstrated that FOs treatment could ameliorate AAPH-induced oxidative stress in HepG2 cells via Nrf2 signalling.http://www.sciencedirect.com/science/article/pii/S1756464616301554Feruloyl oligosaccharides (FOs)Nrf2AAPHHepG2 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Huijuan Zhang Jing Wang Yingli Liu Lingxiao Gong Baoguo Sun |
spellingShingle |
Huijuan Zhang Jing Wang Yingli Liu Lingxiao Gong Baoguo Sun Wheat bran feruloyl oligosaccharides ameliorate AAPH-induced oxidative stress in HepG2 cells via Nrf2 signalling Journal of Functional Foods Feruloyl oligosaccharides (FOs) Nrf2 AAPH HepG2 |
author_facet |
Huijuan Zhang Jing Wang Yingli Liu Lingxiao Gong Baoguo Sun |
author_sort |
Huijuan Zhang |
title |
Wheat bran feruloyl oligosaccharides ameliorate AAPH-induced oxidative stress in HepG2 cells via Nrf2 signalling |
title_short |
Wheat bran feruloyl oligosaccharides ameliorate AAPH-induced oxidative stress in HepG2 cells via Nrf2 signalling |
title_full |
Wheat bran feruloyl oligosaccharides ameliorate AAPH-induced oxidative stress in HepG2 cells via Nrf2 signalling |
title_fullStr |
Wheat bran feruloyl oligosaccharides ameliorate AAPH-induced oxidative stress in HepG2 cells via Nrf2 signalling |
title_full_unstemmed |
Wheat bran feruloyl oligosaccharides ameliorate AAPH-induced oxidative stress in HepG2 cells via Nrf2 signalling |
title_sort |
wheat bran feruloyl oligosaccharides ameliorate aaph-induced oxidative stress in hepg2 cells via nrf2 signalling |
publisher |
Elsevier |
series |
Journal of Functional Foods |
issn |
1756-4646 |
publishDate |
2016-08-01 |
description |
Protective effects of wheat bran feruloyl oligosaccharides (FOs) against 2, 2′-Azobis (2-methylpropionamidine) dihydrochloride (AAPH)-induced oxidative stress in HepG2 cells were investigated. The cytoprotective effects of FOs against oxidative challenge were determined by cell viability, intracellular reactive oxygen species (ROS) generation, and lactate dehydrogenase (LDH) leakage. The antioxidant activities of FOs were investigated by determining the activities and mRNA expression levels of phase II detoxifying/antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx). FOs concentrations had a positive correlation with cell viability and a negative correlation with the LDH activity. FOs treatment increased the activities and mRNA levels of SOD, CAT, and GPx. Moreover, the nuclear factor E2 related factor (Nrf2) protein expression in the FOs group positively correlated with the SOD, CAT, and GPx protein levels. Thus the results demonstrated that FOs treatment could ameliorate AAPH-induced oxidative stress in HepG2 cells via Nrf2 signalling. |
topic |
Feruloyl oligosaccharides (FOs) Nrf2 AAPH HepG2 |
url |
http://www.sciencedirect.com/science/article/pii/S1756464616301554 |
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