Prime editing in mice reveals the essentiality of a single base in driving tissue-specific gene expression

Prime editing in mice reveals the essentiality of a single base in driving tissue-specific gene expression

Abstract Background Most single nucleotide variants (SNVs) occur in noncoding sequence where millions of transcription factor binding sites (TFBS) reside. Here, a comparative analysis of CRISPR-mediated homology-directed repair (HDR) versus the recently reported prime editing 2 (PE2) system was carr...

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Bibliographic Details
Main Authors: Pan Gao, Qing Lyu, Amr R. Ghanam, Cicera R. Lazzarotto, Gregory A. Newby, Wei Zhang, Mihyun Choi, Orazio J. Slivano, Kevin Holden, John A. Walker, Anastasia P. Kadina, Rob J. Munroe, Christian M. Abratte, John C. Schimenti, David R. Liu, Shengdar Q. Tsai, Xiaochun Long, Joseph M. Miano
Format: Article
Language:English
Published: BMC 2021-03-01
Series:Genome Biology
Subjects:
Mouse
CRISPR
Prime editing
Genome editing
Transcription
Gene expression
Online Access:https://doi.org/10.1186/s13059-021-02304-3

Internet

https://doi.org/10.1186/s13059-021-02304-3

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