RNA-Seq workflow: gene-level exploratory analysis and differential expression [version 2; referees: 2 approved]
Here we walk through an end-to-end gene-level RNA-Seq differential expression workflow using Bioconductor packages. We will start from the FASTQ files, show how these were aligned to the reference genome, and prepare a count matrix which tallies the number of RNA-seq reads/fragments within each gene...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
F1000 Research Ltd
2016-11-01
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| Series: | F1000Research |
| Subjects: | |
| Online Access: | https://f1000research.com/articles/4-1070/v2 |