Tuberomics: a molecular profiling for the adaption of edible fungi (Tuber magnatum Pico) to different natural environments
Abstract Background Truffles are symbiotic fungi that develop underground in association with plant roots, forming ectomycorrhizae. They are primarily known for the organoleptic qualities of their hypogeous fruiting bodies. Primarily, Tuber magnatum Pico is a greatly appreciated truffle species main...
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doaj-48e1a3077d5b4acbaf50a618210307352021-01-31T16:12:01ZengBMCBMC Genomics1471-21642020-01-0121112510.1186/s12864-020-6522-3Tuberomics: a molecular profiling for the adaption of edible fungi (Tuber magnatum Pico) to different natural environmentsFederico Vita0Beatrice Giuntoli1Edoardo Bertolini2Cosimo Taiti3Elettra Marone4Chiara D’Ambrosio5Emanuela Trovato6Danilo Sciarrone7Mariosimone Zoccali8Raffaella Balestrini9Andrea Scaloni10Luigi Mondello11Stefano Mancuso12Massimo Alessio13Amedeo Alpi14Dipartimento di Scienze e Tecnologie Agrarie, Alimentari, Ambientali e Forestali (DAGRI), University of FlorenceDepartment of Biology, Università di PisaInstitute of Life Sciences, Scuola Superiore Sant’AnnaDipartimento di Scienze e Tecnologie Agrarie, Alimentari, Ambientali e Forestali (DAGRI), University of FlorenceFaculty of Biosciences and Technologies for Agriculture Food and Environment, University of TeramoProteomics and Mass Spectrometry Laboratory, I.S.P.A.A.M., National Research CouncilChromaleont Srl, c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences Polo Annunziata, University of MessinaDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, Polo Annunziata, University of MessinaDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, Polo Annunziata, University of MessinaNational Research Council of Italy, Institute for Sustainable Plant Protection (CNR-IPSP)Proteomics and Mass Spectrometry Laboratory, I.S.P.A.A.M., National Research CouncilChromaleont Srl, c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences Polo Annunziata, University of MessinaDipartimento di Scienze e Tecnologie Agrarie, Alimentari, Ambientali e Forestali (DAGRI), University of FlorenceDivision of Genetics and Cell Biology, IRCCS-Ospedale San RaffaeleA.R.E.A. FoundationAbstract Background Truffles are symbiotic fungi that develop underground in association with plant roots, forming ectomycorrhizae. They are primarily known for the organoleptic qualities of their hypogeous fruiting bodies. Primarily, Tuber magnatum Pico is a greatly appreciated truffle species mainly distributed in Italy and Balkans. Its price and features are mostly depending on its geographical origin. However, the genetic variation within T. magnatum has been only partially investigated as well as its adaptation to several environments. Results Here, we applied an integrated omic strategy to T. magnatum fruiting bodies collected during several seasons from three different areas located in the North, Center and South of Italy, with the aim to distinguish them according to molecular and biochemical traits and to verify the impact of several environments on these properties. With the proteomic approach based on two-dimensional electrophoresis (2-DE) followed by mass spectrometry, we were able to identify proteins specifically linked to the sample origin. We further associated the proteomic results to an RNA-seq profiling, which confirmed the possibility to differentiate samples according to their source and provided a basis for the detailed analysis of genes involved in sulfur metabolism. Finally, geographical specificities were associated with the set of volatile compounds produced by the fruiting bodies, as quantitatively and qualitatively determined through proton transfer reaction-mass spectrometry (PTR-MS) and gas-chromatography-mass spectrometry (GC-MS). In particular, a partial least squares-discriminant analysis (PLS-DA) model built from the latter data was able to return high confidence predictions of sample source. Conclusions Results provide a characterization of white fruiting bodies by a wide range of different molecules, suggesting the role for specific compounds in the responses and adaptation to distinct environments.https://doi.org/10.1186/s12864-020-6522-3Tuber magnatum PicoSulfur compoundsEnvironmentVolatile organic compoundsIntegrated approach |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Federico Vita Beatrice Giuntoli Edoardo Bertolini Cosimo Taiti Elettra Marone Chiara D’Ambrosio Emanuela Trovato Danilo Sciarrone Mariosimone Zoccali Raffaella Balestrini Andrea Scaloni Luigi Mondello Stefano Mancuso Massimo Alessio Amedeo Alpi |
spellingShingle |
Federico Vita Beatrice Giuntoli Edoardo Bertolini Cosimo Taiti Elettra Marone Chiara D’Ambrosio Emanuela Trovato Danilo Sciarrone Mariosimone Zoccali Raffaella Balestrini Andrea Scaloni Luigi Mondello Stefano Mancuso Massimo Alessio Amedeo Alpi Tuberomics: a molecular profiling for the adaption of edible fungi (Tuber magnatum Pico) to different natural environments BMC Genomics Tuber magnatum Pico Sulfur compounds Environment Volatile organic compounds Integrated approach |
author_facet |
Federico Vita Beatrice Giuntoli Edoardo Bertolini Cosimo Taiti Elettra Marone Chiara D’Ambrosio Emanuela Trovato Danilo Sciarrone Mariosimone Zoccali Raffaella Balestrini Andrea Scaloni Luigi Mondello Stefano Mancuso Massimo Alessio Amedeo Alpi |
author_sort |
Federico Vita |
title |
Tuberomics: a molecular profiling for the adaption of edible fungi (Tuber magnatum Pico) to different natural environments |
title_short |
Tuberomics: a molecular profiling for the adaption of edible fungi (Tuber magnatum Pico) to different natural environments |
title_full |
Tuberomics: a molecular profiling for the adaption of edible fungi (Tuber magnatum Pico) to different natural environments |
title_fullStr |
Tuberomics: a molecular profiling for the adaption of edible fungi (Tuber magnatum Pico) to different natural environments |
title_full_unstemmed |
Tuberomics: a molecular profiling for the adaption of edible fungi (Tuber magnatum Pico) to different natural environments |
title_sort |
tuberomics: a molecular profiling for the adaption of edible fungi (tuber magnatum pico) to different natural environments |
publisher |
BMC |
series |
BMC Genomics |
issn |
1471-2164 |
publishDate |
2020-01-01 |
description |
Abstract Background Truffles are symbiotic fungi that develop underground in association with plant roots, forming ectomycorrhizae. They are primarily known for the organoleptic qualities of their hypogeous fruiting bodies. Primarily, Tuber magnatum Pico is a greatly appreciated truffle species mainly distributed in Italy and Balkans. Its price and features are mostly depending on its geographical origin. However, the genetic variation within T. magnatum has been only partially investigated as well as its adaptation to several environments. Results Here, we applied an integrated omic strategy to T. magnatum fruiting bodies collected during several seasons from three different areas located in the North, Center and South of Italy, with the aim to distinguish them according to molecular and biochemical traits and to verify the impact of several environments on these properties. With the proteomic approach based on two-dimensional electrophoresis (2-DE) followed by mass spectrometry, we were able to identify proteins specifically linked to the sample origin. We further associated the proteomic results to an RNA-seq profiling, which confirmed the possibility to differentiate samples according to their source and provided a basis for the detailed analysis of genes involved in sulfur metabolism. Finally, geographical specificities were associated with the set of volatile compounds produced by the fruiting bodies, as quantitatively and qualitatively determined through proton transfer reaction-mass spectrometry (PTR-MS) and gas-chromatography-mass spectrometry (GC-MS). In particular, a partial least squares-discriminant analysis (PLS-DA) model built from the latter data was able to return high confidence predictions of sample source. Conclusions Results provide a characterization of white fruiting bodies by a wide range of different molecules, suggesting the role for specific compounds in the responses and adaptation to distinct environments. |
topic |
Tuber magnatum Pico Sulfur compounds Environment Volatile organic compounds Integrated approach |
url |
https://doi.org/10.1186/s12864-020-6522-3 |
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