Molecular basis for the PAM expansion and fidelity enhancement of an evolved Cas9 nuclease.
Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems have been harnessed as powerful genome editing tools in diverse organisms. However, the off-target effects and the protospacer adjacent motif (PAM) compatibility restrict the therapeutic applications of these systems. Rec...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2019-10-01
|
Series: | PLoS Biology |
Online Access: | https://doi.org/10.1371/journal.pbio.3000496 |