A cell-free nanobody engineering platform rapidly generates SARS-CoV-2 neutralizing nanobodies

A cell-free nanobody engineering platform rapidly generates SARS-CoV-2 neutralizing nanobodies

Faster, higher throughput antibody engineering methods are needed. Here the authors present CeVICA, a cell-free nanobody engineering platform using ribosome display and computational clustering analysis for in vitro selection; they use this to develop nanobodies against the RBD of SARS-CoV-2 spike p...

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Bibliographic Details
Main Authors: Xun Chen, Matteo Gentili, Nir Hacohen, Aviv Regev
Format: Article
Language:English
Published: Nature Publishing Group 2021-09-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-021-25777-z
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spelling doaj-56b2b7942e424f41b5a4614d612ffcf22021-09-19T11:50:10ZengNature Publishing GroupNature Communications2041-17232021-09-0112111410.1038/s41467-021-25777-zA cell-free nanobody engineering platform rapidly generates SARS-CoV-2 neutralizing nanobodiesXun Chen0Matteo Gentili1Nir Hacohen2Aviv Regev3Klarman Cell Observatory, Broad Institute of MIT and HarvardBroad Institute of MIT and HarvardBroad Institute of MIT and HarvardKlarman Cell Observatory, Broad Institute of MIT and HarvardFaster, higher throughput antibody engineering methods are needed. Here the authors present CeVICA, a cell-free nanobody engineering platform using ribosome display and computational clustering analysis for in vitro selection; they use this to develop nanobodies against the RBD of SARS-CoV-2 spike protein.https://doi.org/10.1038/s41467-021-25777-z
collection DOAJ
language English
format Article
sources DOAJ
author Xun Chen
Matteo Gentili
Nir Hacohen
Aviv Regev
spellingShingle Xun Chen
Matteo Gentili
Nir Hacohen
Aviv Regev
A cell-free nanobody engineering platform rapidly generates SARS-CoV-2 neutralizing nanobodies
Nature Communications
author_facet Xun Chen
Matteo Gentili
Nir Hacohen
Aviv Regev
author_sort Xun Chen
title A cell-free nanobody engineering platform rapidly generates SARS-CoV-2 neutralizing nanobodies
title_short A cell-free nanobody engineering platform rapidly generates SARS-CoV-2 neutralizing nanobodies
title_full A cell-free nanobody engineering platform rapidly generates SARS-CoV-2 neutralizing nanobodies
title_fullStr A cell-free nanobody engineering platform rapidly generates SARS-CoV-2 neutralizing nanobodies
title_full_unstemmed A cell-free nanobody engineering platform rapidly generates SARS-CoV-2 neutralizing nanobodies
title_sort cell-free nanobody engineering platform rapidly generates sars-cov-2 neutralizing nanobodies
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2021-09-01
description Faster, higher throughput antibody engineering methods are needed. Here the authors present CeVICA, a cell-free nanobody engineering platform using ribosome display and computational clustering analysis for in vitro selection; they use this to develop nanobodies against the RBD of SARS-CoV-2 spike protein.
url https://doi.org/10.1038/s41467-021-25777-z
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AT matteogentili acellfreenanobodyengineeringplatformrapidlygeneratessarscov2neutralizingnanobodies
AT nirhacohen acellfreenanobodyengineeringplatformrapidlygeneratessarscov2neutralizingnanobodies
AT avivregev acellfreenanobodyengineeringplatformrapidlygeneratessarscov2neutralizingnanobodies
AT xunchen cellfreenanobodyengineeringplatformrapidlygeneratessarscov2neutralizingnanobodies
AT matteogentili cellfreenanobodyengineeringplatformrapidlygeneratessarscov2neutralizingnanobodies
AT nirhacohen cellfreenanobodyengineeringplatformrapidlygeneratessarscov2neutralizingnanobodies
AT avivregev cellfreenanobodyengineeringplatformrapidlygeneratessarscov2neutralizingnanobodies
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