Purification of antioxidant peptides of Moringa oleifera seeds and their protective effects on H2O2 oxidative damaged Chang liver cells
Eight novel and three known antioxidant peptides isolated from protein hydrolysate of Moringa oleifera seeds, using ultrafiltration, anion-exchange chromatography, gel filtration chromatography and reversed phase high performance liquid chromatography (RP-HPLC). The novel peptides were identified as...
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doaj-5906a7a111cd4f29aa7757540116df262021-04-30T07:17:56ZengElsevierJournal of Functional Foods1756-46462020-01-0164103698Purification of antioxidant peptides of Moringa oleifera seeds and their protective effects on H2O2 oxidative damaged Chang liver cellsLi-li Liang0Shi-ying Cai1Min Gao2Xue-mei Chu3Xiao-yang Pan4Kai-Kai Gong5Cheng-wei Xiao6Yin Chen7Yu-qin Zhao8Bin Wang9Kun-lai Sun10Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, ChinaZhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, ChinaZhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, ChinaZhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, ChinaZhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, ChinaCancer Research Institute, Binzhou Medical University Hospital, Yellow River Second Road 661, Binzhou 256603, ChinaResearch and Development Center, Hainan Herui Pharmaceutical Co., Ltd, Haikou 570311, ChinaZhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, ChinaZhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, ChinaZhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, ChinaZhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, China; Corresponding author.Eight novel and three known antioxidant peptides isolated from protein hydrolysate of Moringa oleifera seeds, using ultrafiltration, anion-exchange chromatography, gel filtration chromatography and reversed phase high performance liquid chromatography (RP-HPLC). The novel peptides were identified as Gly-Tyr (GY), Pro-Phe-Glu (PFE), Tyr-Thr-Arg (YTR), Phe-Gly (FG), Gln-Tyr (QY), Ile-Asn (IN), Ser-Phe (SF), Ser-Pro (SP), and the known peptides were identified as Tyr-Phe-Glu (YFE), Ile-Tyr (IY) and Leu-Tyr (LY), respectively, using protein amino acid sequence analyzer and electrospray ionization-mass spectrometry. All the eleven peptides exhibited strong scavenging activities on 2,2-Diphenyl-1picrylhydrazyl radical (DPPH·) (EC50 2.28, 1.60, 1.77, 2.15, 0.97, 1.30, 0.75, 0.91, 1.21, 0.79, and 1.37 mg/L, respectively) and 2,2-azino-bis (3-ethylbenzothia zoline-6-sulfonic acid) diammonium salt radical (ABTS·+) (EC50 1.03, 0.84, 0.95, 0.65, 0.37, 0.54, 0.33, 0.36, 0.67, 0.32 and 0.38 mg/mL, respectively). In addition, SF and QY showed protective effects on oxidative damage Chang liver cells induced by H2O2, and the contents of aspartate aminotransferase (ALT), alanine aminotransferase (AST) and malondialdehyde (MDA) decreased with the increasing concentrations of SF and QY. Furthermore, SF and QY could significantly increase the levels of superoxide dismutase (SOD) and catalase (CAT), thereby effectively scavenge reactive oxygen species (ROS) in H2O2-induced oxidative damaged Chang liver cells (P < 0.05). These results suggested that SF and QY had the ability to protect Chang liver cells from oxidative stress damage and might serve as potential antioxidants or oxidative damage cytoprotective agents used in pharmaceutical and health food industries.http://www.sciencedirect.com/science/article/pii/S175646461930622XMoringa oleifera seedsAntioxidant peptidesOxidative damage protectionChang liver cells |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Li-li Liang Shi-ying Cai Min Gao Xue-mei Chu Xiao-yang Pan Kai-Kai Gong Cheng-wei Xiao Yin Chen Yu-qin Zhao Bin Wang Kun-lai Sun |
spellingShingle |
Li-li Liang Shi-ying Cai Min Gao Xue-mei Chu Xiao-yang Pan Kai-Kai Gong Cheng-wei Xiao Yin Chen Yu-qin Zhao Bin Wang Kun-lai Sun Purification of antioxidant peptides of Moringa oleifera seeds and their protective effects on H2O2 oxidative damaged Chang liver cells Journal of Functional Foods Moringa oleifera seeds Antioxidant peptides Oxidative damage protection Chang liver cells |
author_facet |
Li-li Liang Shi-ying Cai Min Gao Xue-mei Chu Xiao-yang Pan Kai-Kai Gong Cheng-wei Xiao Yin Chen Yu-qin Zhao Bin Wang Kun-lai Sun |
author_sort |
Li-li Liang |
title |
Purification of antioxidant peptides of Moringa oleifera seeds and their protective effects on H2O2 oxidative damaged Chang liver cells |
title_short |
Purification of antioxidant peptides of Moringa oleifera seeds and their protective effects on H2O2 oxidative damaged Chang liver cells |
title_full |
Purification of antioxidant peptides of Moringa oleifera seeds and their protective effects on H2O2 oxidative damaged Chang liver cells |
title_fullStr |
Purification of antioxidant peptides of Moringa oleifera seeds and their protective effects on H2O2 oxidative damaged Chang liver cells |
title_full_unstemmed |
Purification of antioxidant peptides of Moringa oleifera seeds and their protective effects on H2O2 oxidative damaged Chang liver cells |
title_sort |
purification of antioxidant peptides of moringa oleifera seeds and their protective effects on h2o2 oxidative damaged chang liver cells |
publisher |
Elsevier |
series |
Journal of Functional Foods |
issn |
1756-4646 |
publishDate |
2020-01-01 |
description |
Eight novel and three known antioxidant peptides isolated from protein hydrolysate of Moringa oleifera seeds, using ultrafiltration, anion-exchange chromatography, gel filtration chromatography and reversed phase high performance liquid chromatography (RP-HPLC). The novel peptides were identified as Gly-Tyr (GY), Pro-Phe-Glu (PFE), Tyr-Thr-Arg (YTR), Phe-Gly (FG), Gln-Tyr (QY), Ile-Asn (IN), Ser-Phe (SF), Ser-Pro (SP), and the known peptides were identified as Tyr-Phe-Glu (YFE), Ile-Tyr (IY) and Leu-Tyr (LY), respectively, using protein amino acid sequence analyzer and electrospray ionization-mass spectrometry. All the eleven peptides exhibited strong scavenging activities on 2,2-Diphenyl-1picrylhydrazyl radical (DPPH·) (EC50 2.28, 1.60, 1.77, 2.15, 0.97, 1.30, 0.75, 0.91, 1.21, 0.79, and 1.37 mg/L, respectively) and 2,2-azino-bis (3-ethylbenzothia zoline-6-sulfonic acid) diammonium salt radical (ABTS·+) (EC50 1.03, 0.84, 0.95, 0.65, 0.37, 0.54, 0.33, 0.36, 0.67, 0.32 and 0.38 mg/mL, respectively). In addition, SF and QY showed protective effects on oxidative damage Chang liver cells induced by H2O2, and the contents of aspartate aminotransferase (ALT), alanine aminotransferase (AST) and malondialdehyde (MDA) decreased with the increasing concentrations of SF and QY. Furthermore, SF and QY could significantly increase the levels of superoxide dismutase (SOD) and catalase (CAT), thereby effectively scavenge reactive oxygen species (ROS) in H2O2-induced oxidative damaged Chang liver cells (P < 0.05). These results suggested that SF and QY had the ability to protect Chang liver cells from oxidative stress damage and might serve as potential antioxidants or oxidative damage cytoprotective agents used in pharmaceutical and health food industries. |
topic |
Moringa oleifera seeds Antioxidant peptides Oxidative damage protection Chang liver cells |
url |
http://www.sciencedirect.com/science/article/pii/S175646461930622X |
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