Age-related disease association of endogenous γ-H2AX foci in mononuclear cells derived from leukapheresis.
The phosphorylated form of histone H2AX (γ-H2AX) forms immunohistochemically detectable foci at DNA double strand breaks. In peripheral blood mononuclear cells (PBMCs) derived from leukapheresis from patients enrolled in the Baltimore Longitudinal Study of Aging, γ-H2AX foci increased in a linear fa...
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2012-01-01
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doaj-5fa6ec8695b148b685347cf6683e52062020-11-24T21:32:22ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0179e4572810.1371/journal.pone.0045728Age-related disease association of endogenous γ-H2AX foci in mononuclear cells derived from leukapheresis.Shepherd H SchurmanChristopher A DunnRebecca GreavesBinbing YuLuigi FerrucciDeborah L CroteauMichael M SeidmanVilhelm A BohrThe phosphorylated form of histone H2AX (γ-H2AX) forms immunohistochemically detectable foci at DNA double strand breaks. In peripheral blood mononuclear cells (PBMCs) derived from leukapheresis from patients enrolled in the Baltimore Longitudinal Study of Aging, γ-H2AX foci increased in a linear fashion with regards to age, peaking at ~57 years. The relationship between the frequency of γ-H2AX foci and age-related pathologies was assessed. We found a statistically significant (p = 0.023) 50% increase in foci in PBMCs derived from patients with a known history of vitamin D deficiency. In addition, there were trends toward increased γ-H2AX foci in patients with cataracts (34% increase, p<0.10) and in sleep apnea patients (44%, p<0.10). Among patients ≥57 y/o, we found a significant (p = 0.037) 36% increase in the number of γ-H2AX foci/cell for patients with hypertension compared to non-hypertensive patients. Our results support a role for increased DNA damage in the morbidity of age-related diseases. γ -H2AX may be a biomarker for human morbidity in age-related diseases.http://europepmc.org/articles/PMC3448703?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shepherd H Schurman Christopher A Dunn Rebecca Greaves Binbing Yu Luigi Ferrucci Deborah L Croteau Michael M Seidman Vilhelm A Bohr |
spellingShingle |
Shepherd H Schurman Christopher A Dunn Rebecca Greaves Binbing Yu Luigi Ferrucci Deborah L Croteau Michael M Seidman Vilhelm A Bohr Age-related disease association of endogenous γ-H2AX foci in mononuclear cells derived from leukapheresis. PLoS ONE |
author_facet |
Shepherd H Schurman Christopher A Dunn Rebecca Greaves Binbing Yu Luigi Ferrucci Deborah L Croteau Michael M Seidman Vilhelm A Bohr |
author_sort |
Shepherd H Schurman |
title |
Age-related disease association of endogenous γ-H2AX foci in mononuclear cells derived from leukapheresis. |
title_short |
Age-related disease association of endogenous γ-H2AX foci in mononuclear cells derived from leukapheresis. |
title_full |
Age-related disease association of endogenous γ-H2AX foci in mononuclear cells derived from leukapheresis. |
title_fullStr |
Age-related disease association of endogenous γ-H2AX foci in mononuclear cells derived from leukapheresis. |
title_full_unstemmed |
Age-related disease association of endogenous γ-H2AX foci in mononuclear cells derived from leukapheresis. |
title_sort |
age-related disease association of endogenous γ-h2ax foci in mononuclear cells derived from leukapheresis. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2012-01-01 |
description |
The phosphorylated form of histone H2AX (γ-H2AX) forms immunohistochemically detectable foci at DNA double strand breaks. In peripheral blood mononuclear cells (PBMCs) derived from leukapheresis from patients enrolled in the Baltimore Longitudinal Study of Aging, γ-H2AX foci increased in a linear fashion with regards to age, peaking at ~57 years. The relationship between the frequency of γ-H2AX foci and age-related pathologies was assessed. We found a statistically significant (p = 0.023) 50% increase in foci in PBMCs derived from patients with a known history of vitamin D deficiency. In addition, there were trends toward increased γ-H2AX foci in patients with cataracts (34% increase, p<0.10) and in sleep apnea patients (44%, p<0.10). Among patients ≥57 y/o, we found a significant (p = 0.037) 36% increase in the number of γ-H2AX foci/cell for patients with hypertension compared to non-hypertensive patients. Our results support a role for increased DNA damage in the morbidity of age-related diseases. γ -H2AX may be a biomarker for human morbidity in age-related diseases. |
url |
http://europepmc.org/articles/PMC3448703?pdf=render |
work_keys_str_mv |
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