Quantifying autophagy using novel LC3B and p62 TR-FRET assays.

Autophagy is a cellular mechanism that can generate energy for cells or clear misfolded or aggregated proteins, and upregulating this process has been proposed as a therapeutic approach for neurodegenerative diseases. Here we describe a novel set of LC3B-II and p62 time-resolved fluorescence resonan...

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Bibliographic Details
Main Authors: Alberto Bresciani, Maria Carolina Spiezia, Roberto Boggio, Cristina Cariulo, Anja Nordheim, Roberta Altobelli, Kirsten Kuhlbrodt, Celia Dominguez, Ignacio Munoz-Sanjuan, John Wityak, Valentina Fodale, Deanna M Marchionini, Andreas Weiss
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2018-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC5858923?pdf=render