A Split-Luciferase Reporter Recognizing GFP and mCherry Tags to Facilitate Studies of Protein–Protein Interactions

A Split-Luciferase Reporter Recognizing GFP and mCherry Tags to Facilitate Studies of Protein–Protein Interactions

The use of fluorescently-tagged proteins in microscopy has become routine, and anti-GFP (Green fluorescent protein) affinity matrices are increasingly used in proteomics protocols. However, some protein–protein interactions assays, such as protein complementation assays (PCA), require recloning of e...

Full description

Bibliographic Details
Main Authors: Mehdi Moustaqil, Akshay Bhumkar, Laura Gonzalez, Lisa Raoul, Dominic J. B. Hunter, Pascal Carrive, Emma Sierecki, Yann Gambin
Format: Article
Language:English
Published: MDPI AG 2017-12-01
Series:International Journal of Molecular Sciences
Subjects:
protein–protein interaction
split-luciferase
universal reporter
Leishmania tarentolae cell-free
Online Access:https://www.mdpi.com/1422-0067/18/12/2681

Internet

https://www.mdpi.com/1422-0067/18/12/2681

Similar Items