Kinesin-4 KIF21B is a potent microtubule pausing factor
Microtubules are dynamic polymers that in cells can grow, shrink or pause, but the factors that promote pausing are poorly understood. Here, we show that the mammalian kinesin-4 KIF21B is a processive motor that can accumulate at microtubule plus ends and induce pausing. A few KIF21B molecules are s...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
eLife Sciences Publications Ltd
2017-03-01
|
Series: | eLife |
Subjects: | |
Online Access: | https://elifesciences.org/articles/24746 |
id |
doaj-6f94d5e6bfaf4dc0957d5bb79b6a6008 |
---|---|
record_format |
Article |
spelling |
doaj-6f94d5e6bfaf4dc0957d5bb79b6a60082021-05-05T13:19:56ZengeLife Sciences Publications LtdeLife2050-084X2017-03-01610.7554/eLife.24746Kinesin-4 KIF21B is a potent microtubule pausing factorWilhelmina E van Riel0Ankit Rai1https://orcid.org/0000-0002-1569-0919Sarah Bianchi2Eugene A Katrukha3Qingyang Liu4Albert JR Heck5https://orcid.org/0000-0002-2405-4404Casper C Hoogenraad6Michel O Steinmetz7Lukas C Kapitein8https://orcid.org/0000-0001-9418-6739Anna Akhmanova9https://orcid.org/0000-0002-9048-8614Cell Biology, Department of Biology, Faculty of Science, Utrecht University, Utrecht, NetherlandsCell Biology, Department of Biology, Faculty of Science, Utrecht University, Utrecht, NetherlandsLaboratory of Biomolecular Research, Department of Biology and Chemistry, Paul Scherrer Institut, Villigen PSI, SwitzerlandCell Biology, Department of Biology, Faculty of Science, Utrecht University, Utrecht, NetherlandsCell Biology, Department of Biology, Faculty of Science, Utrecht University, Utrecht, NetherlandsBiomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research, Utrecht Institute for Pharmaceutical Sciences and The Netherlands Proteomics Centre, Utrecht University, Utrecht, NetherlandsCell Biology, Department of Biology, Faculty of Science, Utrecht University, Utrecht, NetherlandsLaboratory of Biomolecular Research, Department of Biology and Chemistry, Paul Scherrer Institut, Villigen PSI, SwitzerlandCell Biology, Department of Biology, Faculty of Science, Utrecht University, Utrecht, NetherlandsCell Biology, Department of Biology, Faculty of Science, Utrecht University, Utrecht, NetherlandsMicrotubules are dynamic polymers that in cells can grow, shrink or pause, but the factors that promote pausing are poorly understood. Here, we show that the mammalian kinesin-4 KIF21B is a processive motor that can accumulate at microtubule plus ends and induce pausing. A few KIF21B molecules are sufficient to induce strong growth inhibition of a microtubule plus end in vitro. This property depends on non-motor microtubule-binding domains located in the stalk region and the C-terminal WD40 domain. The WD40-containing KIF21B tail displays preference for a GTP-type over a GDP-type microtubule lattice and contributes to the interaction of KIF21B with microtubule plus ends. KIF21B also contains a motor-inhibiting domain that does not fully block the interaction of the protein with microtubules, but rather enhances its pause-inducing activity by preventing KIF21B detachment from microtubule tips. Thus, KIF21B combines microtubule-binding and regulatory activities that together constitute an autonomous microtubule pausing factor.https://elifesciences.org/articles/24746microtubule dynamicsKinesinEB1Kinesin-4KIF21Bpausing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wilhelmina E van Riel Ankit Rai Sarah Bianchi Eugene A Katrukha Qingyang Liu Albert JR Heck Casper C Hoogenraad Michel O Steinmetz Lukas C Kapitein Anna Akhmanova |
spellingShingle |
Wilhelmina E van Riel Ankit Rai Sarah Bianchi Eugene A Katrukha Qingyang Liu Albert JR Heck Casper C Hoogenraad Michel O Steinmetz Lukas C Kapitein Anna Akhmanova Kinesin-4 KIF21B is a potent microtubule pausing factor eLife microtubule dynamics Kinesin EB1 Kinesin-4 KIF21B pausing |
author_facet |
Wilhelmina E van Riel Ankit Rai Sarah Bianchi Eugene A Katrukha Qingyang Liu Albert JR Heck Casper C Hoogenraad Michel O Steinmetz Lukas C Kapitein Anna Akhmanova |
author_sort |
Wilhelmina E van Riel |
title |
Kinesin-4 KIF21B is a potent microtubule pausing factor |
title_short |
Kinesin-4 KIF21B is a potent microtubule pausing factor |
title_full |
Kinesin-4 KIF21B is a potent microtubule pausing factor |
title_fullStr |
Kinesin-4 KIF21B is a potent microtubule pausing factor |
title_full_unstemmed |
Kinesin-4 KIF21B is a potent microtubule pausing factor |
title_sort |
kinesin-4 kif21b is a potent microtubule pausing factor |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2017-03-01 |
description |
Microtubules are dynamic polymers that in cells can grow, shrink or pause, but the factors that promote pausing are poorly understood. Here, we show that the mammalian kinesin-4 KIF21B is a processive motor that can accumulate at microtubule plus ends and induce pausing. A few KIF21B molecules are sufficient to induce strong growth inhibition of a microtubule plus end in vitro. This property depends on non-motor microtubule-binding domains located in the stalk region and the C-terminal WD40 domain. The WD40-containing KIF21B tail displays preference for a GTP-type over a GDP-type microtubule lattice and contributes to the interaction of KIF21B with microtubule plus ends. KIF21B also contains a motor-inhibiting domain that does not fully block the interaction of the protein with microtubules, but rather enhances its pause-inducing activity by preventing KIF21B detachment from microtubule tips. Thus, KIF21B combines microtubule-binding and regulatory activities that together constitute an autonomous microtubule pausing factor. |
topic |
microtubule dynamics Kinesin EB1 Kinesin-4 KIF21B pausing |
url |
https://elifesciences.org/articles/24746 |
work_keys_str_mv |
AT wilhelminaevanriel kinesin4kif21bisapotentmicrotubulepausingfactor AT ankitrai kinesin4kif21bisapotentmicrotubulepausingfactor AT sarahbianchi kinesin4kif21bisapotentmicrotubulepausingfactor AT eugeneakatrukha kinesin4kif21bisapotentmicrotubulepausingfactor AT qingyangliu kinesin4kif21bisapotentmicrotubulepausingfactor AT albertjrheck kinesin4kif21bisapotentmicrotubulepausingfactor AT casperchoogenraad kinesin4kif21bisapotentmicrotubulepausingfactor AT michelosteinmetz kinesin4kif21bisapotentmicrotubulepausingfactor AT lukasckapitein kinesin4kif21bisapotentmicrotubulepausingfactor AT annaakhmanova kinesin4kif21bisapotentmicrotubulepausingfactor |
_version_ |
1721462004286226432 |