Low-Level Mouse DNA in Conditioned Medium Generates False Positive Cross-Species Contamination Results in Human Organoid Cultures

Low-Level Mouse DNA in Conditioned Medium Generates False Positive Cross-Species Contamination Results in Human Organoid Cultures

Cell line authentication is critical for preventing the use of mixed or misidentified cell lines in research. Current efforts include short tandem repeat (STR) analysis and PCR-based assays to detect mixed species cultures. Using PCR analysis with mouse-specific primers, we identified contaminating...

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Bibliographic Details
Main Authors: Margaret S. Bohm, Michael K. Dame, Joseph Boyd, Kevin Su, Angeline Wu, Durga Attili, Vi Chu, Justin A. Colacino, Jason R. Spence
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-11-01
Series:Frontiers in Cell and Developmental Biology
Subjects:
cell-free DNA
cfDNA
colonoid
conditioned medium
enteroid
murine contamination
Online Access:https://www.frontiersin.org/articles/10.3389/fcell.2020.587107/full

Internet

https://www.frontiersin.org/articles/10.3389/fcell.2020.587107/full

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