Rapid and Effective Generation of Nanobody Based CARs using PCR and Gibson Assembly

Rapid and Effective Generation of Nanobody Based CARs using PCR and Gibson Assembly

Recent approval of chimeric antigen receptor (CAR) T cell therapy by the European Medicines Agency (EMA)/Federal and Drug Administration (FDA) and the remarkable results of CAR T clinical trials illustrate the curative potential of this therapy. While CARs against a multitude of different antigens a...

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Bibliographic Details
Main Authors: Stijn De Munter, Alexander Van Parys, Layla Bral, Joline Ingels, Glenn Goetgeluk, Sarah Bonte, Melissa Pille, Lore Billiet, Karin Weening, Annick Verhee, Jose Van der Heyden, Tom Taghon, Georges Leclercq, Tessa Kerre, Jan Tavernier, Bart Vandekerckhove
Format: Article
Language:English
Published: MDPI AG 2020-01-01
Series:International Journal of Molecular Sciences
Subjects:
nanobody
vhh
chimeric antigen receptor
car t cell
cd33
cd20
pcr
gibson assembly
nanocar
Online Access:https://www.mdpi.com/1422-0067/21/3/883

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https://www.mdpi.com/1422-0067/21/3/883

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