Impact of quenching failure of Cy dyes in differential gel electrophoresis.

BACKGROUND: Differential gel electrophoresis (DIGE) is a technology widely used for protein expression analysis. It is based on labelling with fluorescent Cy dyes. In comparative fluorescence gel electrophoresis experiments, however, unspecific labelling using N-hydroxy-succinimide-ester-based label...

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Bibliographic Details
Main Authors: Weiqun Wang, Doreen Ackermann, Anna-Maria Mehlich, Simone König
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3068157?pdf=render