Improved yield of high molecular weight DNA coincides with increased microbial diversity access from iron oxide cemented sub-surface clay environments.

Despite over three decades of progress, extraction of high molecular weight (HMW) DNA from high clay soils or iron oxide cemented clay has remained challenging. HMW DNA is desirable for next generation sequencing as it yields the most comprehensive coverage. Several DNA extraction procedures were co...

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Bibliographic Details
Main Authors: Richard A Hurt, Michael S Robeson, Migun Shakya, James G Moberly, Tatiana A Vishnivetskaya, Baohua Gu, Dwayne A Elias
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4102596?pdf=render