Highly Efficient Ultracentrifugation-free Chromatographic Purification of Recombinant AAV Serotype 9

Highly Efficient Ultracentrifugation-free Chromatographic Purification of Recombinant AAV Serotype 9

Recombinant adeno-associated virus serotype 9 (rAAV9) can specifically transduce muscle and neuronal tissues; thus, rAAV9 can potentially be used in gene therapy. However, rAAV9 is the most challenging rAAV serotype to purify. Traditionally, rAAV9 has been purified by ultracentrifugation, which is n...

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Bibliographic Details
Main Authors: Taro Tomono, Yukihiko Hirai, Hironori Okada, Yoshitaka Miyagawa, Kumi Adachi, Shuhei Sakamoto, Yasuhiro Kawano, Hideto Chono, Junichi Mineno, Akiko Ishii, Takashi Shimada, Masafumi Onodera, Akira Tamaoka, Takashi Okada
Format: Article
Language:English
Published: Elsevier 2018-12-01
Series:Molecular Therapy: Methods & Clinical Development
Online Access:http://www.sciencedirect.com/science/article/pii/S2329050118301116

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http://www.sciencedirect.com/science/article/pii/S2329050118301116

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