Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis
Insertions/Deletions (InDels) remain an untapped source of protein diversity in laboratory evolution. The method TRIAD generates libraries of random variants with short in-frame InDels using transposons, allowing a comparison of their evolutionary potential with widely-used point mutant libraries.
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2020-07-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-020-17061-3 |