Functional Trade-Offs in Promiscuous Enzymes Cannot Be Explained by Intrinsic Mutational Robustness of the Native Activity.

by Miriam Kaltenbach, Stephane Emond, Florian Hollfelder, Nobuhiko Tokuriki
Published 2016-10-01

UMI-linked consensus sequencing enables phylogenetic analysis of directed evolution

by Paul Jannis Zurek, Philipp Knyphausen, Katharina Neufeld, Ahir Pushpanath, Florian Hollfelder
Published 2020-11-01

Reverse evolution leads to genotypic incompatibility despite functional and active site convergence

by Miriam Kaltenbach, Colin J Jackson, Eleanor C Campbell, Florian Hollfelder, Nobuhiko Tokuriki
Published 2015-08-01

Monodisperse Water-in-Oil-in-Water (W/O/W) Double Emulsion Droplets as Uniform Compartments for High-Throughput Analysis via Flow Cytometry

by Jing Yan, Wolfgang-Andreas C. Bauer, Martin Fischlechner, Florian Hollfelder, Clemens F. Kaminski, Wilhelm T. S. Huck
Published 2013-12-01

Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis

by Stephane Emond, Maya Petek, Emily J. Kay, Brennen Heames, Sean R. A. Devenish, Nobuhiko Tokuriki, Florian Hollfelder
Published 2020-07-01

Combinatorial Synthesis of Structurally Diverse Triazole-Bridged Flavonoid Dimers and Trimers

by Tze Han Sum, Tze Jing Sum, Warren R. J. D. Galloway, Súil Collins, David G. Twigg, Florian Hollfelder, David R. Spring
Published 2016-09-01

Mutations in Mll2, an H3K4 methyltransferase, result in insulin resistance and impaired glucose tolerance in mice.

by Michelle Goldsworthy, Nathan L Absalom, David Schröter, Helen C Matthews, Debora Bogani, Lee Moir, Anna Long, Christopher Church, Alison Hugill, Quentin M Anstee, Rob Goldin, Mark Thursz, Florian Hollfelder, Roger D Cox
Published 2013-01-01

Investigating host-microbiome interactions by droplet based microfluidics

by Alexandra S. Tauzin, Mariana Rangel Pereira, Liisa D. Van Vliet, Pierre-Yves Colin, Elisabeth Laville, Jeremy Esque, Sandrine Laguerre, Bernard Henrissat, Nicolas Terrapon, Vincent Lombard, Marion Leclerc, Joël Doré, Florian Hollfelder, Gabrielle Potocki-Veronese
Published 2020-10-01

Engineering the protein dynamics of an ancestral luciferase

by Andrea Schenkmayerova, Gaspar P. Pinto, Martin Toul, Martin Marek, Lenka Hernychova, Joan Planas-Iglesias, Veronika Daniel Liskova, Daniel Pluskal, Michal Vasina, Stephane Emond, Mark Dörr, Radka Chaloupkova, David Bednar, Zbynek Prokop, Florian Hollfelder, Uwe T. Bornscheuer, Jiri Damborsky
Published 2021-06-01