Protocol for a mouse CNS demyelination model induced by a combination of HSV-1 and IL-2

Protocol for a mouse CNS demyelination model induced by a combination of HSV-1 and IL-2

Summary: Multiple sclerosis (MS) is an autoimmune demyelinating disease of the central nervous system. Although viruses have been suggested to be a contributing environmental factor, conventional experimental MS mouse models do not account for this aspect. Here, we describe a mouse model to induce a...

Full description

Bibliographic Details
Main Authors: Satoshi Hirose, Kati Tormanen, Mihoko Kato, Jack Yu, Homayon Ghiasi
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Model organisms
Neuroscience
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720302744
id doaj-96ed676624124f93ae8fc68a44622c1d
record_format Article
spelling doaj-96ed676624124f93ae8fc68a44622c1d2021-03-22T12:53:01ZengElsevierSTAR Protocols2666-16672021-03-0121100287Protocol for a mouse CNS demyelination model induced by a combination of HSV-1 and IL-2Satoshi Hirose0Kati Tormanen1Mihoko Kato2Jack Yu3Homayon Ghiasi4Center for Neurobiology & Vaccine Development, Ophthalmology Research, Department of Surgery, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Corresponding authorCenter for Neurobiology & Vaccine Development, Ophthalmology Research, Department of Surgery, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USADepartment of Biology, Pomona College, Claremont, CA 91711, USACenter for Neurobiology & Vaccine Development, Ophthalmology Research, Department of Surgery, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USACenter for Neurobiology & Vaccine Development, Ophthalmology Research, Department of Surgery, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Corresponding authorSummary: Multiple sclerosis (MS) is an autoimmune demyelinating disease of the central nervous system. Although viruses have been suggested to be a contributing environmental factor, conventional experimental MS mouse models do not account for this aspect. Here, we describe a mouse model to induce and evaluate demyelinating disease with both a viral and an immune component via ocular infection with a recombinant herpes simplex virus expressing murine interleukin-2.For complete details on the use and execution of this protocol, please refer to Hirose et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166720302744Model organismsNeuroscience
collection DOAJ
language English
format Article
sources DOAJ
author Satoshi Hirose
Kati Tormanen
Mihoko Kato
Jack Yu
Homayon Ghiasi
spellingShingle Satoshi Hirose
Kati Tormanen
Mihoko Kato
Jack Yu
Homayon Ghiasi
Protocol for a mouse CNS demyelination model induced by a combination of HSV-1 and IL-2
STAR Protocols
Model organisms
Neuroscience
author_facet Satoshi Hirose
Kati Tormanen
Mihoko Kato
Jack Yu
Homayon Ghiasi
author_sort Satoshi Hirose
title Protocol for a mouse CNS demyelination model induced by a combination of HSV-1 and IL-2
title_short Protocol for a mouse CNS demyelination model induced by a combination of HSV-1 and IL-2
title_full Protocol for a mouse CNS demyelination model induced by a combination of HSV-1 and IL-2
title_fullStr Protocol for a mouse CNS demyelination model induced by a combination of HSV-1 and IL-2
title_full_unstemmed Protocol for a mouse CNS demyelination model induced by a combination of HSV-1 and IL-2
title_sort protocol for a mouse cns demyelination model induced by a combination of hsv-1 and il-2
publisher Elsevier
series STAR Protocols
issn 2666-1667
publishDate 2021-03-01
description Summary: Multiple sclerosis (MS) is an autoimmune demyelinating disease of the central nervous system. Although viruses have been suggested to be a contributing environmental factor, conventional experimental MS mouse models do not account for this aspect. Here, we describe a mouse model to induce and evaluate demyelinating disease with both a viral and an immune component via ocular infection with a recombinant herpes simplex virus expressing murine interleukin-2.For complete details on the use and execution of this protocol, please refer to Hirose et al. (2020).
topic Model organisms
Neuroscience
url http://www.sciencedirect.com/science/article/pii/S2666166720302744
work_keys_str_mv AT satoshihirose protocolforamousecnsdemyelinationmodelinducedbyacombinationofhsv1andil2
AT katitormanen protocolforamousecnsdemyelinationmodelinducedbyacombinationofhsv1andil2
AT mihokokato protocolforamousecnsdemyelinationmodelinducedbyacombinationofhsv1andil2
AT jackyu protocolforamousecnsdemyelinationmodelinducedbyacombinationofhsv1andil2
AT homayonghiasi protocolforamousecnsdemyelinationmodelinducedbyacombinationofhsv1andil2
_version_ 1724207580860907520

Similar Items