Novel Method for High-Throughput Full-Length IGHV-D-J Sequencing of the Immune Repertoire from Bulk B-Cells with Single-Cell Resolution

Novel Method for High-Throughput Full-Length IGHV-D-J Sequencing of the Immune Repertoire from Bulk B-Cells with Single-Cell Resolution

Efficient and accurate high-throughput DNA sequencing of the adaptive immune receptor repertoire (AIRR) is necessary to study immune diversity in healthy subjects and disease-related conditions. The high complexity and diversity of the AIRR coupled with the limited amount of starting material, which...

Full description

Bibliographic Details
Main Authors: Stefano Vergani, Ilya Korsunsky, Andrea Nicola Mazzarello, Gerardo Ferrer, Nicholas Chiorazzi, Davide Bagnara
Format: Article
Language:English
Published: Frontiers Media S.A. 2017-09-01
Series:Frontiers in Immunology
Subjects:
next generation sequencing
immunoglobulin repertoire
Illumina Miseq sequencing
VDJ rearrangement
cDNA library
unique molecular identifier
Online Access:http://journal.frontiersin.org/article/10.3389/fimmu.2017.01157/full

Internet

http://journal.frontiersin.org/article/10.3389/fimmu.2017.01157/full

Similar Items