Red-Shifted FRET Biosensors for High-Throughput Fluorescence Lifetime Screening

Red-Shifted FRET Biosensors for High-Throughput Fluorescence Lifetime Screening

We have developed fluorescence resonance energy transfer (FRET) biosensors with red-shifted fluorescent proteins (FP), yielding improved characteristics for time-resolved (lifetime) fluorescence measurements. In comparison to biosensors with green and red FRET pairs (GFP/RFP), FPs that emit at longe...

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Bibliographic Details
Main Authors: Tory M. Schaaf, Ang Li, Benjamin D. Grant, Kurt Peterson, Samantha Yuen, Prachi Bawaskar, Evan Kleinboehl, Ji Li, David D. Thomas, Gregory D. Gillispie
Format: Article
Language:English
Published: MDPI AG 2018-10-01
Series:Biosensors
Subjects:
time-resolved FRET
biosensor
drug screening
SERCA
small-molecule
high-throughput
plate reader
fluorescence
fluorescent proteins
Online Access:https://www.mdpi.com/2079-6374/8/4/99

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https://www.mdpi.com/2079-6374/8/4/99

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