Next generation sequencing to define prokaryotic and fungal diversity in the bovine rumen.

A combination of Sanger and 454 sequences of small subunit rRNA loci were used to interrogate microbial diversity in the bovine rumen of 12 cows consuming a forage diet. Observed bacterial species richness, based on the V1-V3 region of the 16S rRNA gene, was between 1,903 to 2,432 species-level oper...

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Bibliographic Details
Main Authors: Derrick E Fouts, Sebastian Szpakowski, Janaki Purushe, Manolito Torralba, Richard C Waterman, Michael D MacNeil, Leeson J Alexander, Karen E Nelson
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3492333?pdf=render