Isolation and Characterization of Multi-Protein Complexes Enriched in the K-Cl Co-transporter 2 From Brain Plasma Membranes

Isolation and Characterization of Multi-Protein Complexes Enriched in the K-Cl Co-transporter 2 From Brain Plasma Membranes

Kcc2 plays a critical role in determining the efficacy of synaptic inhibition, however, the cellular mechanisms neurons use to regulate its membrane trafficking, stability and activity are ill-defined. To address these issues, we used affinity purification to isolate stable multi-protein complexes o...

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Bibliographic Details
Main Authors: Joshua L. Smalley, Georgina Kontou, Catherine Choi, Qiu Ren, David Albrecht, Krithika Abiraman, Miguel A. Rodriguez Santos, Christopher E. Bope, Tarek Z. Deeb, Paul A. Davies, Nicholas J. Brandon, Stephen J. Moss
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-10-01
Series:Frontiers in Molecular Neuroscience
Subjects:
potassium chloride cotransporter 2
proteome
interactome
phosphorylation
protein purification
Online Access:https://www.frontiersin.org/articles/10.3389/fnmol.2020.563091/full

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https://www.frontiersin.org/articles/10.3389/fnmol.2020.563091/full

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