No detectable alloreactive transcriptional responses under standard sample preparation conditions during donor-multiplexed single-cell RNA sequencing of peripheral blood mononuclear cells

No detectable alloreactive transcriptional responses under standard sample preparation conditions during donor-multiplexed single-cell RNA sequencing of peripheral blood mononuclear cells

Abstract Background Single-cell RNA sequencing (scRNA-seq) provides high-dimensional measurements of transcript counts in individual cells. However, high assay costs and artifacts associated with analyzing samples across multiple sequencing runs limit the study of large numbers of samples. Sample mu...

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Bibliographic Details
Main Authors: Christopher S. McGinnis, David A. Siegel, Guorui Xie, George Hartoularos, Mars Stone, Chun J. Ye, Zev J. Gartner, Nadia R. Roan, Sulggi A. Lee
Format: Article
Language:English
Published: BMC 2021-01-01
Series:BMC Biology
Subjects:
scRNA-seq
Sample multiplexing
Sample preparation
Alloreactivity
PBMCs
Online Access:https://doi.org/10.1186/s12915-020-00941-x

Internet

https://doi.org/10.1186/s12915-020-00941-x

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