Precision oncology using a limited number of cells: optimization of whole genome amplification products for sequencing applications

Precision oncology using a limited number of cells: optimization of whole genome amplification products for sequencing applications

Abstract Background Sequencing analysis of circulating tumor cells (CTCs) enables “liquid biopsy” to guide precision oncology strategies. However, this requires low-template whole genome amplification (WGA) that is prone to errors and biases from uneven amplifications. Currently, quality control (QC...

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Bibliographic Details
Main Authors: Shonan Sho, Colin M. Court, Paul Winograd, Sangjun Lee, Shuang Hou, Thomas G. Graeber, Hsian-Rong Tseng, James S. Tomlinson
Format: Article
Language:English
Published: BMC 2017-07-01
Series:BMC Cancer
Subjects:
Precision oncology
Whole genome amplification
Single-cell sequencing
Next generation sequencing
Multiple displacement amplification
Online Access:http://link.springer.com/article/10.1186/s12885-017-3447-6

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http://link.springer.com/article/10.1186/s12885-017-3447-6

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