Ultrasensitive Imaging of Ca2+ Dynamics in Pancreatic Acinar Cells of Yellow Cameleon-Nano Transgenic Mice

Ultrasensitive Imaging of Ca2+ Dynamics in Pancreatic Acinar Cells of Yellow Cameleon-Nano Transgenic Mice

Yellow Cameleons are genetically encoded Ca2+ indicators in which cyan and yellow fluorescent proteins and calmodulin work together as a fluorescence (Förster) resonance energy transfer Ca2+-sensor probe. To achieve ultrasensitive Ca2+ imaging for low resting Ca2+ or small Ca2+ transients in various...

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Bibliographic Details
Main Authors: Yusuke Oshima, Takeshi Imamura, Atsuko Shintani, Hiroko Kajiura-Kobayashi, Terumasa Hibi, Takeharu Nagai, Shigenori Nonaka, Tomomi Nemoto
Format: Article
Language:English
Published: MDPI AG 2014-11-01
Series:International Journal of Molecular Sciences
Subjects:
Yellow Cameleon
Ca2+ indicator
Ca2+ imaging
Förster resonance energy transfer (FRET)
genetically encoded Ca2+ indicators (GECI)
Yellow Cameleon-Nano (YC-Nano)
two-photon excitation fluorescence microscopy
live-cell imaging
exocytotsis
acinar cell
Online Access:http://www.mdpi.com/1422-0067/15/11/19971

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http://www.mdpi.com/1422-0067/15/11/19971

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